| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibody | Capture antibody and horseradish peroxidase (HRP) labelled detection antibody, both specific for rat cytochrome c and rat cytochrome c standard (Cat. No. DYC897, DuoSet IC, Rat/Mouse Total Cytochrome c kit), were from R&D systems (McKinley Place NE, MN, USA). Anti-HRP antibody (Cat. No. A00619) was obtained from GenScript (Piscataway, NJ, USA). | Get A Quote |
Cytochrome c is an intermembrane mitochondrial protein that is released to the bloodstream following mitochondrial injury.,We developed an electrochemiluminescence immunoassay to measure cytochrome c in human and rat plasma, which showed high sensitivity with broad dynamic range (2-1200 ng/mL in humans and 5-500 ng/mL in rat) and high assay reproducibility (inter-assay coefficient <6% in humans and <10% in rat). In patients after blunt trauma, plasma cytochrome c directly correlated with injury severity. In rats after cardiac resuscitation, plasma cytochrome c inversely correlated with survival and responsiveness to mitochondrial protective interventions.,The cytochrome c assays herein presented have high s... More
